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Gene Synthesis> | … Escherichia coli The coding sequence of the suckerin-12 gene was synthesized (GenScript) and sub-cloned using NdeI and BamHI into the pET15b expression plasmid (Novagen) with an amino terminal hexa-histidine tag. The … | Get A Quote |
The suckerin family of proteins, identified from the squid sucker ring teeth assembly, offers unique mechanical properties and potential advantages over other natural biomaterials. In this study, a small suckerin isoform, suckerin-12, is used to create enzymatically crosslinked, macro-scale hydrogels. Upon exposure to specific salt conditions, suckerin-12 hydrogels contracted into a condensed state where mechanical properties are found to be modulated by the salt anion present. The rate of contraction is found to correlate well with the kosmotropic arm of the Hofmeister anion series. However, the observed changes in hydrogel mechanical properties are better explained by the ability of the salt t... More