Products/Services Used | Details | Operation |
---|---|---|
PCR and Cloning> | … The genes encoding the affibody molecules from selections were subcloned into the E. coli expression vector pET22b (GenScript Biotech Corp, NJ) under control of a T7 promoter and as genetic fusions with a C-terminal sortase A site, followed by ABD and a hexahistidine tag … | Get A Quote |
Development of new affinity proteins using combinatorial protein engineering is today established for generation of monoclonal antibodies and also essential for discovery of binders that are based on non-immunoglobulin proteins. Phage display is most frequently used, but yeast display is becoming increasingly popular, partly due to the option of utilizing fluorescence-activated cell sorting (FACS) for isolation of new candidates. Escherichia coli has several valuable properties for library applications and in particular the high transformation efficiency. The use of various autotransporters and intimins for secretion and anchoring on the outer membrane have shown promising results and particularly for direc... More