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Gene Synthesis> | … No. AND74811.1). For this, 50 μl of chemically competent Escherichia coli BL21 cells were thawed on ice, mixed with 0.08 μg plasmid DNA containing VC-CTBX gene (Genscript, Piscataway, NJ, USA) in sterile water and incubated on ice for 30 min … | Get A Quote |
Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with high affinity and specificity. CT is the main virulence factor of Vibrio cholerae and the causative agent of the eponymous disease. For the selected aptamers, dissociation constants in the low nanomolar range (23-56 nM) were determined by fluorescence-based affinity chromatography and cross-reactivity against related proteins was evaluated by direct enzyme-linked aptamer assay (ELAA). Ap... More