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Nucleic Acid Purification & Analysis> | … Diagram of competitive binding between imidazole and 6-His tag to bind Ni-NTA beads The eight stored samples (1 lysate, 1 flow-through, 5 wash steps, and 2 elutions) for each purification conducted were analyzed using SDS-PAGE (GenScript) at 150 volts for 40 minutes … | Get A Quote |
Prostate specific antigen (PSA) is the most significant biomarker for the screening of prostate cancer in human serum. However, most methods for the detection of PSA often require major laboratories, precisely analytical instruments and complicated operations. Currently, the design and development of satisfying electrochemical biosensors based on biomimetic materials (e.g. synthetic receptors) and nanotechnology is highly desired. Thus, we focused on the combination of molecular recognition and versatile nanomaterials in electrochemical devices for advancing their analytical performance and robustness. Herein, by using the present prepared multifunctional hydroxyl pillar[5]arene@gold nanoparticles@gra... More