Products/Services Used | Details | Operation |
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Catalog Antibody> | … Aldrich, St. Louis, MO); anti-calnexin (Enzo Life Sciences, Inc., Farmingdale, NY); anti-MBP (GenScript, Piscataway, NJ); anti-FLAG M2 (Sigma-Aldrich, St. Louis, MO); anti-His (Sigma- Aldrich, St. Louis, MO); anti-GM130 (BD Biosciences, San Jose, CA); anti-GRP78 (Santa Cruz … | Get A Quote |
Sumoylation regulates a wide range of essential cellular functions, many of which are associated with activities in the nucleus. Although there is also emerging evidence for the involvement of the small ubiquitin-related modifier (SUMO) at intracellular membranes, the mechanisms by which sumoylation is regulated at membranes is largely unexplored. In this study, we report that the SUMO-specific isopeptidase, SENP2, uniquely associates with intracellular membranes. Using in vivo analyses and in vitro binding assays, we show that SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix that promotes direct membrane binding. Furthermore, we demonstrate that SENP2 b... More