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Gene Synthesis> | ...DNA encoding Ig2 (domains 5 and 6 of the gelation factor ABP-120 of Dictyostelium discoideum) (McCoy et al., 1999) was synthesized by Genscript and cloned by PCR into pET28a(+)tev, which was modified to contain an N-terminal 6xHis-tag and a tobacco etch virus (TEV) cleavage site... | Get A Quote |
The Lon AAA+ protease (LonA) plays important roles in protein homeostasis and regulation of diverse biological processes. LonA behaves as a homomeric hexamer in the presence of magnesium (Mg(2+)) and performs ATP-dependent proteolysis. However, it is also found that LonA can carry out Mg(2+)-dependent degradation of unfolded protein substrate in an ATP-independent manner. Here we show that in the presence of Mg(2+) LonA forms a non-secluded hexameric barrel with prominent openings, which explains why Mg(2+)-activated LonA can operate as a diffusion-based chambered protease to degrade unstructured protein and peptide substrates efficiently in the absence of ATP. A 1.85 Å crystal structure of Mg(2+)-activated pr... More