Products/Services Used | Details | Operation |
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Gene Synthesis> | Gene encoding full-length mjADPGK (Uniprot: Q58999, residues 4-462) was optimized for E. coli expression system, synthesized (GenScript) and cloned without expression/purification tag into pET24d plasmid using NcoI/BamHI restriction sites. E | Get A Quote |
ADP-dependent glucokinase (ADPGK) is an alternative novel glucose phosphorylating enzyme in a modified glycolysis pathway of hyperthermophilic Archaea. In contrast to classical ATP-dependent hexokinases, ADPGK utilizes ADP as a phosphoryl group donor. Here, we present a crystal structure of archaeal ADPGK from Methanocaldococcus jannaschii in complex with an inhibitor, 5-iodotubercidin, d-glucose, inorganic phosphate, and a magnesium ion. Detailed analysis of the architecture of the active site allowed for confirmation of the previously proposed phosphorylation mechanism and the crucial role of the invariant arginine residue (Arg197). The crystal structure shows how the phosphate ion, while mimicking a β-phosp... More