Products/Services Used | Details | Operation |
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Mutagenesis Services> | Synthetic genes of human SgK223 αN1-PsK-Cter (residues 932–1406), SgK269 αN1-PsK-Cter (residues 1267–1746), truncated SgK223 αN1 constructs (residues 975–1406) and mutants (GenScript) were cloned into a modified pCOLD IV vector that contains a N-terminal His TEV cleavage tag | Get A Quote |
The mammalian pseudokinase SgK223, and its structurally related homologue SgK269, are oncogenic scaffolds that nucleate the assembly of specific signalling complexes and regulate tyrosine kinase signalling. Both SgK223 and SgK269 form homo- and hetero-oligomers, a mechanism that underpins a diversity of signalling outputs. However, mechanistic insights into SgK223 and SgK269 homo- and heterotypic association are lacking. Here we present the crystal structure of SgK223 pseudokinase domain and its adjacent N- and C-terminal helices. The structure reveals how the N- and C-regulatory helices engage in a novel fold to mediate the assembly of a high-affinity dimer. In addition, we identified regulatory interfaces on ... More