Products/Services Used | Details | Operation |
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Gene Synthesis> | To express the protein in E. coli, the nucleotide sequence encoding Csep1p (locus tag CCS77_2074 on the pICON plasmid, GenBank ID CP021643.1) minus the signal peptide sequence was codon optimized, synthesized, and cloned into the pET151/D-TOPO vector (Invitrogen, Waltham, MA, USA) by GenScript. | Get A Quote |
Colonization of Campylobacter concisus in the gastrointestinal tract can lead to the development of inflammatory bowel disease (IBD). Plasmid-encoded C. concisus-secreted protein 1 (Csep1p ) was recently identified as a putative pathogenicity marker associated with active Crohn’s disease, a clinical form of IBD. Csep1p shows no significant full-length sequence similarity to proteins of known structure, and its role in pathogenesis is not yet known. This study reports a method for extraction of recombinantly expressed Csep1p from Escherichia coli inclusion bodies, refolding, and purification to produce crystallizable protein. Purified recombinant Csep1p behaved as a monomer in solution. Crystals of C... More