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Gene Synthesis> | … 2.2. Cloning and purification of the SPP1 connector. Gene gp6 encoding SPP1 portal was synthesized and cloned into PET3 vector between NhdeI and BamHI by GenScript, Inc. His-tag was inserted into the C-terminal of the connector for purification … | Get A Quote |
Nanopore technology has become a powerful tool in single molecule sensing, and protein nanopores appear to be more advantageous than synthetic counterparts with regards to channel amenability, structure homogeneity, and production reproducibility. However, the diameter of most of the well-studied protein nanopores is too small to allow the passage of protein or peptides that are typically in multiple nanometers scale. The portal channel from bacteriophage SPP1 has a large channel size that allows the translocation of peptides with higher ordered structures. Utilizing single channel conductance assay and optical single molecule imaging, we observed translocation of peptides and quantitatively analyzed ... More