| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … Genes encoding two OmpA variants containing a histidine epitope tag were synthesized by GenScript (Piscataway, NJ, USA) in a pUC57 shuttle vector with flanking NcoI and NotI restriction sites … The gene cassette was synthesized by Genscript and cloned to a shuttle vector … | Get A Quote |
To facilitate the rapid purification of bacterial outer membrane vesicles (OMVs)， we developed two plasmid constructs that utilize a truncated， transmembrane protein to present an exterior histidine repeat sequence. We chose OmpA， a highly abundant porin protein， as the protein scaffold and utilized the lac promoter to allow for inducible control of the epitope-presenting construct. OMVs containing mutant OmpA-His6 were purified directly from Escherichia coli culture media on an immobilized metal affinity chromatography (IMAC) Ni-NTA resin. This enabling technology can be combined with other molecular tools directed at OMV packaging to facilitate the separation of modified/cargo-loaded OMV from their wt... More
