Products/Services Used | Details | Operation |
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Peptide Synthesis> | … Materials and Methods. Peptide Synthesis and Bacterial Cultures. Peptides were synthesized by Genscript (Piscataway Township, NJ, USA) at >80% purity … His-tagged P1 or sP1 peptides (GenScript, Piscataway Township, NJ, USA) were added to the cultures at 0.1 mg/mL … | Get A Quote |
is the primary agent of dental cavities, in large part due to its ability to adhere to teeth and create a molecular scaffold of glucan polysaccharides on the tooth surface. Disrupting the architecture of biofilms could help undermine the establishment of biofilm communities that cause cavities and tooth decay. Here we present a synthetic peptide P1, derived from a tick antifreeze protein, which significantly reduces biofilm formation. Incubating cells with this peptide decreased biofilm biomass by approximately 75% in both a crystal violet microplate assay and an tooth model using saliva-coated hydroxyapatite discs. Bacteria treated with peptide P1 formed irregular biofilms with disconnected aggregates... More