Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | … To produce plant expression constructs, both wild-type (WT) and plant-optimized fragment (LPH-OP) sequences were synthesized and cloned into the pUC57 vector by GenScript Inc. to derive the cloning vectors, pUC-WT-Lum and pUC-LPH-Lum … | Get A Quote |
Lumbrokinases, a group of fibrinolytic enzymes extracted from earthworm, have been widely used to prevent and treat various cardiovascular diseases. They specifically target fibrin to effectively degrade thrombi without major side effects. Plant expression systems are becoming potential alternative expression platforms for producing pharmaceutical proteins. In this work, a lumbrokinase (PI239) was produced from a plant system. Both wild-type (WT) and plant codon-optimized (OP) gene sequences were synthesized and cloned into a geminivirus-based single-vector DNA replicon system. Both vectors were independently expressed in tobacco leaves transiently by agroinfiltration. Overexpressed PI239 resulted in su... More