Products/Services Used | Details | Operation |
---|---|---|
Codon Optimization> | … All restriction, PCR and cloning steps were performed according to standard procedures. 41 The synthetic codon-optimized genes for CMCR-S1 and ADH-A were obtained from GenScript (Piscataway, USA) and Thermo Fisher Scientific (Waltham, USA), respectively … | Get A Quote |
Studying enzymatic reductions of substrates with more than a single keto group is challenging, as the carbonyl reduction can create a vast array of regio- and stereoisomers. If used as reference compounds, regio- and stereopure hydroxy ketides could facilitate the characterization of reductases with unclear regio- and stereoselectivity. We have combined nonenzymatic and enzymatic reduction and oxidation steps to obtain all four regio- and stereoisomers of tert-butyl hydroxyoxohexanoates in high optical purity (enantiomeric ratio (er) of 99 : 1 for the δ-hydroxy-β-keto isomers; er of >97 : 3 for the β-hydroxy-δ-keto isomers). Furthermore, we have prepared seven of the eight possible regioisomer... More