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Peptide Synthesis> | … Synthetic gene encoding the selected protein (without signal peptide) with an N-terminal His6-Ala-Gly-tag and sequence optimized for expression in E.coli, were ordered from GenScript (NJ, USA), amplified by PCR and cloned into the pNIC-CH [19] vector utilizing Ligation … | Get A Quote |
Chitin, a polymer of β(1-4)-linked N-acetylglucosamine found in e.g. arthropods, is a valuable resource that may be used to produce chitosan and chitooligosaccharides, two compounds with considerable industrial and biomedical potential. Deacetylating enzymes may be used to tailor the properties of chitin and its derived products. Here, we describe a novel CE4 enzyme originating from a marine Arthrobacter species (ArCE4A). Crystal structures of this novel deacetylase were determined, with and without bound chitobiose [(GlcNAc)2], and refined to 2.1 ? and 1.6 ?, respectively. In-depth biochemical characterization showed that ArCE4A has broad substrate specificity, with higher activity against lon... More