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Gene Synthesis> | … The human NANOGNB coding sequence was synthesized by GenScript USA and cloned in-frame with a C-terminal V5 tag under the control of a CMV promoter in a GFP co-expressing vector (pSF-CMV-Ub-daGFP AscI, Oxford Genetics #OG244) … | Get A Quote |
The homeobox gene plays a pivotal role in self-renewal and maintenance of pluripotency in human, mouse and other vertebrate embryonic stem cells, and in pluripotent cells of the blastocyst inner cell mass. There is a poorly studied and atypical homeobox locus close to the gene in some mammals which could conceivably be a cryptic paralogue of even though the loci share only 20% homeodomain identity. Here we argue that this gene, , is an extremely divergent duplicate of that underwent radical sequence change in the mammalian lineage. Like , the gene is expressed in pre-implantation embryos of human and cow; unlike , expression is restricted to 8-cell and morula stages, preceding blastocyst for... More