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Proteins, Expression, Isolation and Analysis> | … The endotoxin content of RHPS, determined using the Toxin SensorTM chromogenic LAL endotoxin assay kit (GenScript, NJ, USA), was <0.01 EU/mL … 6237), anti-TRAIL (H-257, sc-7877), and anti-β actin (N-21, sc-131,656) purchased from Santa Cruz Biotechnology (TX, USA) … | Get A Quote |
Rice hull polysaccharides (RHPS) have been reported to activate innate immunity in mice. This study investigated the effects of RHPS on natural killer (NK) cell-mediated cytotoxicity in vitro and the possible underlying anticancer mechanisms in vivo. The results showed that sustained exposure to RHPS increased NK-92MI cell-mediated cytotoxicity in a time- and concentration-dependent manner. In addition, RHPS upregulated the expression of Fas ligand, TNF-related apoptosis-inducing ligand, perforin, and granzyme B of NK-92MI cells and induced the secretion of IFN-γ and TNF-α. In the in vivo experiment, colon cancer CT26-bearing mice were used to investigate the effects of RHPS in cytotoxicity and anti... More