| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … and 100 μg/ml) of AGEs (cat#bs-1158P, Beijing Biosynthesis Biotechnology, China) for 24 h. In some experiments, HPMCs were treated with 50 μg/ml AGEs for 12, 24 and 48 h. We also used the ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit (GenScript Biotech Corp … | Get A Quote |
Epithelial-mesenchymal transition (EMT) in peritoneum was induced during peritoneal dialysis (PD)， which finally caused progressive fibrosis. However， the underlying mechanisms were not well elucidated. We established advanced glycation end-products (AGEs)-induced EMT model using primary human peritoneal mesothelial cells (HPMCs). The working concentration and time of AGEs were optimized. Then the expression and activation signal transducer and activator of transcription 3 (STAT3)， a key factor of EMT in cancer， were detected. The regulation of STAT3 by miRNA was also explored. 50 μg/ml AGEs treatment for 24 h can successfully induce EMT in HPMCs. AGEs treatment upregulated and activated STAT3. miRNA... More
