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Molecular characterization of Streptococcus pneumoniae in children living in southwest China and assessment of a potential protein vaccine， rPfbA.

Vaccine. 2019-01;

YanZiyi, CuiYali, ZhouWei, LiWensheng, TanXi, ChenWen, ZhangJing, JiangYon

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	… gki, recP, spi, xpt, ddl) were amplified by PCR and sequenced by the Sanger method (ABI3730XL, Sangon Biotech, Shanghai, China) according to … Next, rPfbA was purified using a Ni-NTA column, cleared of endotoxins using a polymyxin B column (GenScript, New Jersey, USA …	Get A Quote

摘要

Few children living in southwest China are vaccinated against Streptococcus pneumoniae (S. pneumoniae)， which is an important pathogen in causing high morbidity and high mortality. This study aimed the molecular characterization of S. pneumoniae strains isolated from children and a new vaccine strategy based on a potential protein antigen.