Products/Services Used | Details | Operation |
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Gene Synthesis> | … Journal of Industrial Microbiology & Biotechnology … and PAL/TAL; (2) c-Myc-tag for 4CL and BSMT, and FLAG-tag for VDH (GenScript) … facilitate subsequent 'Sequence- and Ligation-Independent Cloning' (SLIC) procedure using the MultiColi system (GENEVA Biotech; http://www … | Get A Quote |
Since the 1930s, parabens have been employed widely as preservatives in food, pharmaceutical, and personal care products. These alkyl esters of benzoic acid occur naturally in a broad range of plant species, where they are thought to enhance overall fitness through disease resistance and allelopathy. Current manufacture of parabens relies on chemical synthesis and the processing of 4-hydroxybenzoate as a precursor. A variety of bio-based production platforms have targeted 4-hydroxybenzoate for a greener alternative to chemical manufacturing, but parabens have yet to be made in microbes. Here, we deploy the plant enzyme benzoic acid carboxyl methyltransferase together with four additional recombina... More