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Proteins, Expression, Isolation and Analysis> | … was monitored every 10 min using a digital thermometer (ALC-ET06, Shanghai Alcott Biotech Co., Shanghai, China), which was … The endotoxin bioassay was performed in duplicate using a GenScript endotoxin detection kit (ToxinSensor? Chromogenic LAL Endotoxin Assay Kit … | Get A Quote |
Intestinal barrier dysfunction plays a pivotal role in multiorgan dysfunction during heatstroke (HS). Neutrophils are involved in intestinal inflammation and thus dampen the mucosal integrity. Regulatory T cells (Tregs) have been shown to orchestrate neutrophils and thus sustain mucosal integrity in miscellaneous inflammation-related diseases. However, whether Tregs are involved in HS-induced intestinal barrier dysfunction remains unknown. Thus, we investigated whether Tregs could alleviate intestinal barrier dysfunction in mice. We found that HS could induce intestinal injury and mucosal barrier dysfunction 0, 24, and 72 h after heat stress. Flow cytometry revealed an increase of neutrophil infiltrati... More