Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … A 50-repeat poly(PR) plasmid was synthesized by Genscript (Piscataway, NJ) in a pet22b vector with a C-terminal GB1 solubility tag … | Get A Quote |
A microsatellite expansion mutation in C9orf72 is the most common genetic cause of Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). The expansion mutation leads to C9orf72 loss of function, RNA foci formation, and generation of five species of non-AUG RAN translated dipeptide repeat proteins (DPRs), such as poly(GA), poly(GP), poly(GR), poly(PA), and poly(PR). Although one cell can contain more than type of DPRs, information about interplay between different DPR species is limited. Here we show that the combined expression of distinct C9orf72-derived dipeptide repeat species produces cellular outcomes and structural differences that are unique compared to the expression of ... More