Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | Tlg1 was N-terminally tagged with HaloTag (Promega, Madison, WI) using a yeast codon-optimized gene (GenScript, Piscataway, NJ) | Get A Quote |
The endomembrane system consists of the secretory and endocytic pathways, which communicate by transport to and from the trans-Golgi network (TGN). In mammalian cells, the endocytic pathway includes early, late, and recycling endosomes. In budding yeast, different types of endosomes have been described, but the organization of the endocytic pathway has remained unclear. We performed a spatial and temporal analysis of yeast endosomal markers and endocytic cargoes. Our results indicate that the yeast TGN also serves as an early and recycling endosome. In addition, as previously described, yeast contains a late or prevacuolar endosome (PVE). Endocytic cargoes localize to the TGN shortly after inter... More