| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | a, St. Louis, MO). Cellular proteins were collected and recombinant proteins were affinity purified according the High Affinity Ni-NTA Resin Kit protocol (Genscript, China).. Eluted proteins were dialyzed against 20 mM of HEPES (pH 8.0) plus 150 mmol L−1 of NaCl at 4°C and frozen in 10% glycerol at 80 °C. TAT-NLS protein was synthesized directly (Genscript) and used as a control | Get A Quote |
To date， the molecular mechanism underlying constitutive signal transducer and activator of transcription 3 (STAT3) activation in gliomas is largely unclear. In this study， we report that Smad6 is overexpressed in nuclei of glioma cells， which correlates with poor patient survival and regulates STAT3 activity via negatively regulating the Protein Inhibitors of Activated STAT3 (PIAS3). Mechanically， Smad6 interacts directly with PIAS3， and this interaction is mediated through the Mad homology 2 (MH2) domain of Smad6 and the Ring domain of PIAS3. Smad6 recruits Smurf1 to facilitate PIAS3 ubiquitination and degradation， which also depends on the MH2 domain and the PY motif of Smad6. Consequently， Sma... More
