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The IDA3 adapter， required for intraflagellar transport of I1 dynein， is regulated by ciliary length.

Mol. Biol. Cell. 2018;

HunterEmily L，LechtreckKarl，FuGang，HwangJuyeon，LinHuawen，GokhaleAvanti，AlfordLea M，LewisBrian，YamamotoRyosuke，KamiyaRitsu，YangFan，NicastroDaniela，DutcherSusan K，WirschellMaureen，SaleWinfie

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	The APHVIII cassette was inserted into pCP3 with Smal and KpnI to produce the plasmid IC140::GFP-AphVIII. The 3.28-kb NruI – EcoRI fragment from IC140::GFP-APHVIII was excised and subcloned into plasmid pSE280 to produce pCS5.1. A 2.068-kb NruIRsrII fragment containing the SNAP tag in place of GFP in exon 2 was synthesized and cloned into the pUC57-Kan vector (Genscript, Piscataway, NJ) to make clone pCS17.	Get A Quote

摘要

We determined how the ciliary motor I1 dynein is transported. A specialized adapter， IDA3， facilitates I1 dynein attachment to the ciliary transporter called intraflagellar transport (IFT). Loading of IDA3 and I1 dynein on IFT is regulated by ciliary length.