Products/Services Used | Details | Operation |
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Gene Synthesis> | The membranes were blocked in 10% skim milk powder solution, incubated with a 1:2000 dilution of mouse anti RpoS (Santa cruz), Flag-tag (Beyotime), Lon (Sigma), EseB (GenScript, Nanjing, China), RpoB (OriGene) or DnaK (Huabio, Hangzhou, China) antibodies, and finally incubated with a 1:2000 dilution of anti-mouse peroxidase-conjugated IgG secondary antibodies (Sigma). | Get A Quote |
Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry. The pathogen depends on type III and type VI secretion systems (T3/T6SS) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription activator. Here, we performed a Tn-seq-based screen to uncover factors that govern esrB expression. Unexpectedly, we discovered that RpoS antagonizes esrB expression and thereby inhibits production of E. piscicida's T3/T6SS. Using in vitro transcription assays, we showed that RpoS can block RpoD-mediated transcription of esrB. ChIP-seq- and RNA-seq-based profiling, as well as mutational and biochemical analyse... More