Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Full-length IQD5 cDNA plus 6×His Tag in N terminal was synthesized and cloned into expression vector pET30a, expressed and purified by Genscript (https://www.genscript.com/). | Get A Quote |
The dynamic arrangement of cortical microtubules (MTs) plays a pivotal role in controlling cell growth and shape formation in plants, but the mechanisms by which cortical MTs are organized to regulate these processes are not well characterized. In particular, the dynamic behavior of cortical MTs is critical for their spatial organization, yet the molecular mechanisms controlling MT dynamics remain poorly understood. In this study, we used the puzzle piece-shaped pavement cells of Arabidopsis () leaves as a model system in which to study cortical MT organization. We isolated an ethyl methanesulfonate mutant with reduced interdigitation of pavement cells in cotyledons. This line carried a mutation in ()... More