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Gene Synthesis> | ) Briefly, the gene of N-terminal FLAG and C-terminal Myc tagged CDH17 (NM_001144663) was optimized for codon usage for Sf9 and cloned into pUC57 vector by GenScript.As a template, all of the PCR amplification were performed using the gene of N-terminal FLAG and Cterminal Myc tagged CDH17 (NM_001144663) optimized for codon usage for Sf9 and cloned into a pUC57 vector by GenScript. | Get A Quote |
Cadherin-17 (CDH17) is highly expressed in gastric cancer and is thus considered to be a good target for antibody therapy. CDH17 is classified as a nonclassical cadherin, in that it is composed of seven extracellular cadherin domains. We generated anti-CDH17 monoclonal antibodies (mAbs) which recognize the extracellular domain of CDH17. Competitive assay using AGS, a gastric cancer cell line, cells revealed that five selected anti-CDH17 mAbs recognize different epitopes on CDH17. As AGS cells were shown to exhibit broad expression pattern of CDH17 by flow cytometry, we separated three clones with a low (10,000/cell), medium (50,000/cell), and high (200,000/cell) expression level, designati... More