Products/Services Used | Details | Operation |
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Custom Vector Construction> | pCDNA3.1 vectors containing His-tagged TBK1 WT (wild-type), TBK1 K38A (Lys38 mutated to Ala), TBK1 KDD (kinase domain deletion) and HA-tagged wild-type p100 were purchased from GenScript. | Get A Quote |
The NF-κB pathway plays a crucial role in supporting tumor initiation, progression, and radioresistance of tumor cells. However, the role of the NF-κB pathway in radiation-induced anti-tumor host immunity remains unclear. Here we demonstrated that inhibiting the canonical NF-κB pathway dampened the therapeutic effect of ionizing radiation (IR), whereas non-canonical NF-κB deficiency promoted IR-induced anti-tumor immunity. Mechanistic studies revealed that non-canonical NF-κB signaling in dendritic cells (DCs) was activated by the STING sensor-dependent DNA-sensing pathway. By suppressing recruitment of the transcription factor RelA onto the Ifnb promoter, activation of the non-canonical NF-κB ... More