Products/Services Used | Details | Operation |
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Gene Synthesis> | Gene synthesis and cloning was performed by Genscript.His6-tagged genes for the five E. coli proteins rplI (L9), rplM (L13), rpmB (L28), rpmG (L33), and rnpA (C5) were synthesized and cloned into pET-29a(+) vectors using NdeI and XhoI restriction sites (GenScript). | Get A Quote |
mRNAs can fold into complex structures that regulate gene expression. Resolving such structures de novo has remained challenging and has limited our understanding of the prevalence and functions of mRNA structure. We use SHAPE-MaP experiments in living E. coli cells to derive quantitative, nucleotide-resolution structure models for 194 endogenous transcripts encompassing approximately 400 genes. Individual mRNAs have exceptionally diverse architectures, and most contain well-defined structures. Active translation destabilizes mRNA structure in cells. Nevertheless, mRNA structure remains similar between in-cell and cell-free environments, indicating broad potential for structure-mediated gene regulation... More