| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | A synthetic gene encoding the flexible linker including two TAG codons was ordered from GenScript, USA) and cloned into a pET28a+ vector encoding a previous LUMABS variant via restriction ligation cloning using Kpn I and Spe I (New England Biolabs, USA). The DNA construct encoding the linker with flanking TAG codons was produced synthetically (GenScript, USA) and cloned into a previous LUMABS variant using a restriction-ligation approach, replacing the old linker with the linker containing two TAG codons, creating pTAG-LUMABS. | Get A Quote |
Single-step immunoassays that can be performed directly in solution are ideally suited for point-of-care diagnostics. Our group recently developed a new platform of bioluminescent sensor proteins (LUMABS; LUMinescent AntiBody Sensor) that allow antibody detection in blood plasma. Thus far， LUMABS has been limited to the detection of antibodies recognizing natural peptide epitopes. Here， we report the development of semisynthetic LUMABS sensors that recognize nonpeptide epitopes. The non-natural amino acid para-azidophenylalanine was introduced at the position of the original antibody-recognition sites as a chemical handle to enable site-specific conjugation of synthetic epitope molecules coupled to a dibenz... More
