Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | The DNA for LntEco and LntPae expression was synthesized and cloned into the pET28a vector using the restriction sites NdeI and XhoI to produce expression constructs with an N-terminal thrombin-cleavable His6-tag (GenScript, USA). | Get A Quote |
Lipoproteins serve essential roles in the bacterial cell envelope. The posttranslational modification pathway leading to lipoprotein synthesis involves three enzymes. All are potential targets for the development of new antibiotics. Here we report the crystal structure of the last enzyme in the pathway, apolipoprotein N-acyltransferase, Lnt, responsible for adding a third acyl chain to the lipoprotein's invariant diacylated N-terminal cysteine. Structures of Lnt from Pseudomonas aeruginosa and Escherichia coli have been solved; they are remarkably similar. Both consist of a membrane domain on which sits a globular periplasmic domain. The active site resides above the membrane interface where the domains m... More