Products/Services Used | Details | Operation |
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Custom Vector Construction> | The Sara3A mutation consists in the replacement in the Sara protein of S636,S709 and S774 by alanines; introduction of theses mutations into the Sara sequence was done by Genscript UAS Inc. The Sara3A construct, GFP tagged in 50 was inserted into a pUAST vector (DGRC) leading to an N-terminal EGFP fusion. | Get A Quote |
During asymmetric division, fate assignation in daughter cells is mediated by the partition of determinants from the mother. In the fly sensory organ precursor cell, Notch signalling partitions into the pIIa daughter. Notch and its ligand Delta are endocytosed into Sara endosomes in the mother cell and they are first targeted to the central spindle, where they get distributed asymmetrically to finally be dispatched to pIIa. While the processes of endosomal targeting and asymmetry are starting to be understood, the machineries implicated in the final dispatch to pIIa are unknown. We show that Sara binds the PP1c phosphatase and its regulator Sds22. Sara phosphorylation on three specific sites functions a... More