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Expressing accessory proteins in cellulolytic to improve the conversion yield of recalcitrant cellulose.

Biotechnol Biofuels. 2017;

GuoZhong-Peng，DuquesneSophie，BozonnetSophie，NicaudJean-Marc，MartyAlain，O'DonohueMichael Jo

Products/Services Used	Details	Operation
Codon Optimization	The SWO1 gene (GenBank accession code: AJ245918.1), encoding T. reesei swollenin, was synthesized by GenScript (USA) after codon optimization based on the codon bias of Y.lipolytica (Additional file 2), and cloned into the plasmid JMP62UraTEF.Blots were sequentially treated with mouse non-position-specific His-Tag antibody 1:2500 (THE™ from Genscript, Piscataway, NJ, US) and the alkaline phosphatase-conjugated goat anti-mouse IgG. Phosphatase activity was detected by NBT/BCIP as substrate.	Get A Quote

摘要

A recently constructed cellulolytic is able to grow efficiently on an industrial organosolv cellulose pulp， but shows limited ability to degrade crystalline cellulose. In this work， we have further engineered this strain， adding accessory proteins xylanase II (XYNII)， lytic polysaccharide monooxygenase (LPMO)， and swollenin (SWO) from in order to enhance the degradation of recalcitrant substrate.