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Gene Synthesis> | The gene encoding CBLB502 (residues 1–296) was cloned into a modified pCold-sumo vector (pCold-SUMOb) that contains an N-terminal His6 tag, Smt3 Fusion Protein and SUMO Protease 1 cleavage site. The CBLB502 gene was synthesized by Genscript (Piscataway, USA) and amplified by PCR. | Get A Quote |
CBLB502 derived from Salmonella flagellin is a novel agonist of Toll-like receptor 5 (TLR5). It has been shown that CBLB502 can exert high radioprotective efficacy on mice and primates from both GI and hematopoietic syndromes during whole-body irradiation with low toxicity and immunogenicity. However, no effective system has been used to investigate the protective effect of CBLB502 against irradiation and the related mechanism in vitro. In this study, we investigated the radioprotective properties of CBLB502 in HEK293-N-T cells constitutively expressing human TLR5 and NF-κB-dependent luciferase. HEK293-N-T cells were treated with different doses of CBLB502 prior to 60Co-γ ray irradiation. After irradiatio... More