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Conferring cellulose-degrading ability to to facilitate a consolidated bioprocessing approach.

Biotechnol Biofuels. 2017;

GuoZhong-Peng，DuquesneSophie，BozonnetSophie，CiociGianluca，NicaudJean-Marc，MartyAlain，O'DonohueMichael Jo

Products/Services Used	Details	Operation
Gene Synthesis	Considering the challenge to express CBH I proteins in yeast, the gene coding the sequences of CBH I from T. reesei, P. funiculosum, and N. crassa were codonoptimized based on the codon bias of Y. lipolytica, and were synthesized by GenScript (USA) and cloned into the plasmid JMP62UraTEF, JMP62LeuTEF, or JMP62UraTB1his under the control of TEF promoter. Blots were sequentially treated with mouse non position-specific His-Tag antibody 1:2500 (THE™ from Genscript, Piscataway, NJ, USA) and the alkaline phosphatase-conjugated goat anti-mouse IgG (1:10000).	Get A Quote

摘要

， one of the most widely studied "nonconventional" oleaginous yeast species， is unable to grow on cellulose. Recently， we identified and overexpressed two endogenous β-glucosidases in ， thus enabling this yeast to use cello-oligosaccharides as a carbon source for growth. Using this engineered yeast platform， we have now gone further toward building a fully cellulolytic for use in consolidated bioprocessing of cellulose.