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Gene Synthesis> | The UCOE (nt 1,397–2,942 of the HNRPA2B1 gene) along with a ClaI site at the 50 end and nt 2,184–2,378 from pLV-CMV at the 30 end were synthesized by Genscript (Piscataway, NJ, USA). The gene encoding sCD4 (amino acids 1–209 of CD4 followed by GGGSGAGCCPGCCHHHHHH) and the nucleotide sequence encoding the signal peptide of human AAT (amino acids 1–24) with an EcoRI site at the 50 end and nt 76–218 of the open reading frame of sCD4 were synthesized by Genscript. Western blot analysis was performed as previously described.25,37 His-tag ELISA(Genscript) was performed according to the manufacturer’s instructions. | Get A Quote |
HIV entry inhibitors are highly effective in controlling virus replication. We have developed a lentiviral vector that expresses a secreted entry inhibitor, soluble CD4 (sCD4), which binds to the HIV envelope glycoproteins and inactivates the virus. We have shown that sCD4 was secreted from gene-modified CD4 T cells, as well as from human umbilical cord blood-derived CD34 hematopoietic stem/progenitor cells (HSPCs), and protected unmodified HIV target cells from infection in vitro. To investigate the in vivo application of our approach, we injected gene-modified HSPCs into NOD/SCID/γc (NSG) mice. NSG hosts supported multi-lineage differentiation of human gene-modified HSPCs. Upon challenge with HI... More