Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | Genetic constructs coding for the ectodomains of hCD25 and hCD122 (in pCEP4, C-terminal His-tagged) were purchased from Genscript, expressed as above and purified using the TALON metal affinity resin (Clontech). | Get A Quote |
Interleukin-2 (IL-2) is an established therapeutic agent used for cancer immunotherapy. Since treatment efficacy is mediated by CD8 and NK cell activity at the tumour site, considerable efforts have focused on generating variants that expand these subsets systemically, as exemplified by IL-2/antibody complexes and 'superkines'. Here we describe a novel determinant of antitumour activity using fusion proteins consisting of IL-2 and the antibody fragment crystallizable (Fc) region. Generation of long-lived IL-2-Fc variants in which CD25 binding is abolished through mutation effectively prevents unwanted activation of CD25 regulatory T-cells (Tregs) and results in strong expansion of CD25 cytotoxic subsets. Su... More