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PCR Cloning and Subcloning> | The amino acid sequence for SFTB-2 (C. elegans SF3B2) was synthesized by GenScript, Inc. and cloned into a pGEX-6p-1 vector. GST-tagged SFTB-2 was purified similarly as human SF3B2, and dialyzed overnight into 10 mM Na2HPO4, 2 mM KH2PO4, 137 mM NaCl, 2.7 mM KCl, and 1 mM DTT (pH 7.4). | Get A Quote |
Caenorhabditis elegans protein arginine methyltransferases PRMT-7 and PRMT-9 are two evolutionarily conserved enzymes, with distinct orthologs in plants, invertebrates, and vertebrates. Biochemical characterization of these two enzymes reveals that they share much in common with their mammalian orthologs. C. elegans PRMT-7 produces only monomethylarginine (MMA) and preferentially methylates R-X-R motifs in a broad collection of substrates, including human histone peptides and RG-rich peptides. In addition, the activity of the PRMT-7 enzyme is dependent on temperature, the presence of metal ions, and the reducing agent dithiothreitol. C. elegans PRMT-7 has a substrate specificity and a substrate pr... More