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Positron Emission Tomography Imaging with 2-[18F]F-p-Aminobenzoic Acid Detects Staphylococcus aureus Infections and Monitors Drug Response

ACS Infectious Diseases. 2018;

Zhuo Zhang, Alvaro A. Ordonez, Hui Wang, Yong Li, Kayla R Gogarty, Edward AWeinstein, Fereidoon Daryaee, Jonathan Merino, Grace Eunbin Yoon, Alvin S Kalinda,Ronnie C Mease, James N. Iuliano, Peter Smith-Jones, Sanjay K. Jain, and Peter J Tonge

Products/Services Used	Details	Operation
Gene Synthesis	Expression and purification of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK):A pET15b vector carrying the gene for E. coli HPPK in frame with an N-terminal His-tag (GenScript USA Inc.) was transformed into competent E. coli BL21(DE3) cells and plated on Lysogeny Broth (LB) agar containing 100 μg/mL ampicillin.	Get A Quote