Products/Services Used | Details | Operation |
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Gene Synthesis> | Vectors (pcDNA3.1(+)) expressing human codon optimized HA-tagged HSV-1 gM was synthesized by Genscript and myc-tagged HSV-2 gM was obtained from Origene (catalog number VC100874). | Get A Quote |
teins encoded by viruses that impair or shutdown specific host cell functions during replication can be used as probes to identify potential proteins/pathways used in the replication of viruses from other families. We screened nine proteins from herpes simplex virus type 1 (HSV-1) for the ability to enhance or restrict human immunodeficiency virus type 1 (HIV-1) replication. We show that several HSV-1 proteins (glycoprotein M (gM), US3 and UL24) potently restricted the replication of HIV-1. Unlike UL24 and US3, which reduced viral protein synthesis, we observed that gM restriction of HIV-1 occurred through interference of the processing and transport of gp160, resulting in a significantly ... More