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Structure and antagonism of the receptor complex mediated by human TSLP in allergy and asthma

Nature Communications. 2017;

Andrew H. Limper Wang, Bruce Klein, Sho Yamasaki, Bernd Lepenies and Vijayalakshmi Nandakumar, Marcel Wüthrich, Huafeng Theodore J. Kottom, Deanne M. Hebrink, Paige E. Jenson

Products/Services Used	Details	Operation
PCR Cloning and Subcloning	Complementary DNA (cDNA) fragments (Genscript) encoding full-length human TSLPR127A/R130S (NP_149024.1; residue 1–159), human IL-7 (NP_000871.1; residue 1–177) and the extracellular fragments of human TSLPR (NP_071431.2; residue 1–221) and human IL-7Ra (NP_002176.2; residue 1–239) were cloned into the pHLsec and pcDNA4/TO-expression vectors in frame with a C-terminal hexahistidine tag.	Get A Quote