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Fam64a is a novel cell cycle promoter of hypoxic fetal cardiomyocytes in mice

Scientific REPORTS. 2017; 
Ken Hashimoto, Aya Kodama, Takeshi Honda, Akira Hanashima, Yoshihiro Ujihara, Takashi Murayama, Shin-ichiro Nishimatsu & Satoshi Mohri
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Gene Synthesis Coding sequence for VSVG and following SV40 polyadenylation signal sequence was amplified from pFBG-CAG16, and inserted into downstream of polyhedrin promoter of the above vector to yield the final pFastBac1-VSVG-CMV-WPRE vector. WT and mutant Fam64a sequence was PCR amplified from adult mouse cDNA and synthetic gene fragments (GenScript, NJ, USA), respectively, and inserted into pFastBac1-VSVG-CMV-WPRE. For EGFP-tagged (C-terminus) proteins, Get A Quote

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