Fam64a is a novel cell cycle promoter of hypoxic fetal cardiomyocytes in mice
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Coding sequence for VSVG and following SV40 polyadenylation signal sequence was amplified from pFBG-CAG16, and inserted into downstream of polyhedrin promoter of the above vector to yield the final pFastBac1-VSVG-CMV-WPRE vector. WT and mutant Fam64a sequence was PCR amplified from adult mouse cDNA and synthetic gene fragments (GenScript, NJ, USA), respectively, and inserted into pFastBac1-VSVG-CMV-WPRE. For EGFP-tagged (C-terminus) proteins, |
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