| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The DNA insert used to build the tsECR1 knock-in plasmid was chemically synthesized (GenScript USA Inc., Piscataway, NJ) to incorporate the L413H mutation along with a 3 _x0006_Myc epitope tag while also modifying the amino acid coding to prevent recombination events bypassing the ts mutation. Phenotypic regeneration of the new tsECR1 strain was verified by IFA and Western analysis (Fig. S2 | Get A Quote |
