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Gene Synthesis> | Mini-PROTEAN® TGX™ Precast Gels were from BioRad. pCMV6 entry vector containing the ORF corresponding to (Myc-DDK-tagged)-Human WW domain containing oxidoreductase (WWOX), transcript variant 1 was purchased from Origene. pcDNA3.1 containing ORF expressing HA-tagged Homo sapiens jun proto-oncogene (JUN) was purchased from Genscript. | Get A Quote |
The tumor suppressor, WWOX, exhibits regulatory interactions with an array of transcription factors and signaling molecules that are positioned at the well-known crossroads between inflammation and cancer. WWOX is also subject to downregulation by genotoxic environmental exposures, making it of potential interest to the study of lung pathobiology. Knockdown of lung WWOX expression in mice was observed to cause neutrophil influx, and accompanied by a corresponding vascular leak and inflammatory cytokine production. In cultured human alveolar epithelial cells, loss of WWOX expression resulted in increased c-Jun- and IL-8- dependent neutrophil chemotaxis towards cell monolayers. WWOX was observed to directl... More