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PCR Cloning and Subcloning> | . DNA sequences for AlkEB8ΔBapBS-lacZ, AlkEB9_OpaBS-lacZ and AlkEB9_OpaKOlacZ were assembled by Genscript and cloned into eve.p-lacZ.attB vector for further PhiC31 directed genome integration.DNA coding sequence of opa was synthesized (Genscript) in frame with carboxy-terminal OLLAS and 6xHis tags and cloned into the pcDNA3.1(+) mammalian expression vector. | Get A Quote |
The Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) plays a critical role in the specification of founder cells (FCs) in the Drosophila visceral mesoderm (VM) during embryogenesis. Reporter gene and CRISPR/Cas9 deletion analysis reveals enhancer regions in and upstream of the Alk locus that influence tissue-specific expression in the amnioserosa (AS), the VM and the epidermis. By performing high throughput yeast onehybrid screens (Y1H) with a library of Drosophila transcription factors (TFs) we identify Oddpaired (Opa), the Drosophila homologue of the vertebrate Zic family of TFs, as a novel regulator of embryonic Alk expression. Further characterization identifies evolutionarily conserved Opa-b... More