Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … vector (Novagen). Rabbit anti-Strep tag II Polyclonal Antibody Affinity Purified HRP conjugated, mouse anti-his antibody and rabbit anti-mouse AP antibody were purchased from GenScript Inc., Invitrogen and Sigma-Aldrich, respectively … | Get A Quote |
Filoviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there are a lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all know... More