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Proteins, Expression, Isolation and Analysis> | Page 1. ORIGINAL PAPER The mir‐465 family is upregulated with age and attenuates growth hormone signaling in mouse liver Amy E. Elias | Bianca Kun | Ian MC Sabula | Gail Golomb‐Mello | Andrea Cespedes Zablah | Jill A. Kreiling … The medium was replaced with serum‐free culture medium containing 500 ng/ml mouse growth hormone (GenScript, Piscataway, NJ, USA). See the Supporting Information Data S1 for more details. | Get A Quote |
We analyzed the small RNA transcriptome from 5-month-old, 24-month-old, and 36-month-old mouse liver and found 56 miRNAs that changed their expression profile with age. Among these is a cluster of 18 miRNAs that are upregulated between 50- and 1,000-fold at 24 and 36 months of age. This cluster is located in a 60-kb region of the X-chromosome that is devoid of other coding sequences and is part of a lamin-associated domain. Potential targets of the miRNAs in the cluster suggest they may regulate several pathways altered in aging, including the PI3K-Akt pathway. Total transcriptome analyses indicate that expression of several potential genes in the PI3K-Akt pathway that may be targeted by the mir-465 fa... More