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PCR Cloning and Subcloning> | … 2.2. Plasmids, transfection, and lentivirus infection. N-terminal HA-tagged full-length human PEAK1 cDNA (WT) and PEAK1 △Shc and △Crk mutants (Genscript Biotechnology, China) were subcloned into pLVX-IRES-Puro lentiviral vectors (Clontech, USA) … | Get A Quote |
Pseudopodium enriched atypical kinase 1 (PEAK1), a novel non-receptor tyrosine kinase, was recently implicated in cancer pathogenesis. However, its functional role in colorectal cancer (CRC) is not well known. Herein, we demonstrated that PEAK1 was frequently downregulated in CRC and significantly associated with tumor size, differentiation status, metastasis, and clinical stage. PEAK1 overexpression suppressed CRC cell growth, invasion, and metastasis in vitro and in vivo, whereas knockout had the opposite effects. Further evaluation revealed that PEAK1 expression was positively correlated with protein phosphatase 1 regulatory subunit 12B (PPP1R12B) in CRC cell lines and clinical tissues,... More