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Proteins, Expression, Isolation and Analysis> | … After electrophoresis, the peptides were transferred to nitrocellulose membranes with a Mini Trans-Blot system (Bio-Rad, USA) … The recombinant proteins rMdRDH1 and rMdRDH1 C135A were purified by using Ni-NTA Resin (GenScript, China) on the basis of the instructions … | Get A Quote |
Rhodanese homology domains (RHODs) are the structural modules of ubiquitous tertiary that occur in three major evolutionary phyla. Despite the versatile and important physiological functions of RHODs containing proteins, little is known about their invertebrate counterparts. A novel HSP67B2-like single-domain rhodanese homologue, MdRDH1 from Musca domestica, whose expression can be induced by bacterial infection or oxidative stress. Silencing MdRDH1 through RNAi causes important accumulations of reactive oxygen species (ROS) and malondialdehyde (MDA), and increases mortality in the larvae treated with bacterial invasion. The E. coli with MdRDH1 and the mutant MdRDH1 are transformed, with significant r... More